English

All-in-One First-Strand Synthesis MasterMix (with dsDNase)
三代逆转录去基因组DNA一管化预混液

产品货号:
  • EG15133S
产品规格:
100 rxns
产品价格:
¥1380.00

产品介绍

       All-in-One First-Strand Synthesis MasterMix (with dsDNase) 是一款高效、便捷、减少污染的高质量一链 cDNA 合成试剂盒,包含热稳定的 M-MLV GIII Reverse Transcriptase 及其反应 Buffer、RNA 酶抑制剂、dNTPs、Oligo(dT)₂₀VN 和随机引物等一链 cDNA 合成所需的所有组分,仅需加入 RNA 模板和水即可开始反应。使用该逆转录试剂盒获得的 cDNA 主要用于下游 qPCR 实验。

       从细胞中提取的 RNA 往往存在基因组 DNA 污染,如果逆转录前不将其去除,下游 qPCR 反应时基因组 DNA 与 cDNA 会同时被扩增(尤其当引物设计在同一外显子上时),从而影响基因表达定量准确性。本试剂盒采用 dsDNase 高效去除基因组 DNA 污染,dsDNase 能够特异性消化双链 DNA(dsDNA 或 DNA-RNA 杂合链中的 DNA 链), 并且具有热敏感性,在逆转录温度下即可快速不可逆地失活。与传统 使用 DNase I 去除基因组 DNA 污染的方法相比,dsDNase 无需额外加入 EDTA 进行失活,不仅节省实验时间,而且降低了对逆转录反应的抑制。
       可依据基因组污染严重程度选择采用去基因组 DNA 污染与逆转录分开进行的操作方法,或者去基因组污染与逆转录一步法进行的操作方法。

产品组成

组分

规格

All-in-One First-Strand Synthesis MasterMix

400 μl

dsDNase

2×50 μl

10× dsDNase Buffer

200 μl

Nuclease-Free Water

2×1 ml

储运条件

-20℃保存

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Lu KK, Yang H, Liao CY, et al. A transcriptional recognition site within SOS1 coding region controls salt tolerance in Arabidopsis. Dev Cell. Published online June 6, 2025. doi:10.1016/j.devcel.2025.05.010

 

Liu J, Liu G, Cao Y, et al. BNC-rSS, a bivalent subunit nanovaccine affords the cross-protection against Streptococcus agalactiae and Streptococcus iniae infection in tilapia. Int J Biol Macromol. 2023;253(Pt 1):126670. doi:10.1016/j.ijbiomac.2023.126670

 

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Meng H, Ai H, Li D, et al. Bombyx mori UFBP1 regulates apoptosis and promotes BmNPV proliferation by affecting the expression of ER chaperone BmBIP [published correction appears in Int J Biol Macromol. 2025 Mar;292:139214. doi: 10.1016/j.ijbiomac.2024.139214.]. Int J Biol Macromol. 2024;283(Pt 3):137681. doi:10.1016/j.ijbiomac.2024.137681

 

Li W, Liang L, Liu S, et al. CD38 inhibits ferroptosis to promote radiotherapy resistance in nasopharyngeal carcinoma by competitively binding to TRIM21 to stabilize SLC7A11 protein. Int J Biol Macromol. 2025;317(Pt 2):144742. doi:10.1016/j.ijbiomac.2025.144742

 

Wu F, Fan J, Ye X, et al. Unraveling Cadmium Toxicity in Trifolium repens L. Seedling: Insight into Regulatory Mechanisms Using Comparative Transcriptomics Combined with Physiological Analyses. Int J Mol Sci. 2022;23(9):4612. Published 2022 Apr 21. doi:10.3390/ijms23094612

 

Guo F, Jin M, Xie Y, Feng L, Jiang L. Combined application of sucrose and 1-MCP alleviated the senescence of Gynura bicolor DC through regulating hexose accumulation. Plant Physiol Biochem. 2024;212:108745. doi:10.1016/j.plaphy.2024.108745

 

Guo F, Han J, Jin M, Xie Y, Jiang L. Effects of sucrose and 1-MCP on enzymatic and nonenzymatic antioxidants in postharvest Gynura bicolor DC. Plant Physiol Biochem. 2025;219:109319. doi:10.1016/j.plaphy.2024.109319

 

Zhao L, Teng Q, Liu Y, et al. Machine learning-based identification of a novel prognosis-related long noncoding RNA signature for gastric cancer. Front Cell Dev Biol. 2022;10:1017767. Published 2022 Nov 11. doi:10.3389/fcell.2022.1017767

 

Gao Z, Yang X, Chen J, Rausher MD, Shi T. Expression inheritance and constraints on cis- and trans-regulatory mutations underlying lotus color variation. Plant Physiol. 2023;191(3):1662-1683. doi:10.1093/plphys/kiac522

 

Liu J, Zhang B, Zhang Y, et al. Oxidative stress and autophagy-mediated immune patterns and tumor microenvironment infiltration characterization in gastric cancer. Aging (Albany NY). 2023;15(21):12513-12536. doi:10.18632/aging.205194

 

Li M, Li N, Dong Y, et al. Soil intake modifies the gut microbiota and alleviates Th2-type immune response in an ovalbumin-induced asthma mouse model. World Allergy Organ J. 2024;17(4):100897. Published 2024 Apr 14. doi:10.1016/j.waojou.2024.100897

 

Liu R, Huang Y, Li D, et al. Dihydroartemisinin alleviates diethylnitrosamine-induced hepatocarcinogenesis by targeting a novel MAZ/TRIM50 axis. Int Immunopharmacol. 2025;156:114733. doi:10.1016/j.intimp.2025.114733

 

Qin XD, Liang JF, Gan LY, et al. Blockage of polycystin-2 alleviates myocardial ischemia/reperfusion injury by inhibiting autophagy through the Ca2+/Akt/Beclin 1 pathway. Biochim Biophys Acta Mol Cell Res. 2025;1872(2):119892. doi:10.1016/j.bbamcr.2024.119892

 

Sun Z, Liu J, Ge P, et al. Rhein Mitigates Lung Injury in Severe Acute Pancreatitis Through the Inhibition of MARK4-Mediated Microtubule Destabilization. J Inflamm Res. 2025;18:1395-1412. Published 2025 Jan 30. doi:10.2147/JIR.S505049

 

Ban D, GeSang Z, Fan Y, Fu G, Yang H, Si L. Therapeutic potential of compound extract from Dracocephalum Rupestre Hance and Berberidis Radix against Salmonella-induced lamb diarrhea. Sci Rep. 2024;14(1):23789. Published 2024 Oct 11. doi:10.1038/s41598-024-73034-2

 

Ma Y, Wu W, Zhang Y, et al. The Synchronized Progression from Mitosis to Meiosis in Female Primordial Germ Cells between Layers and Broilers. Genes (Basel). 2023;14(4):781. Published 2023 Mar 23. doi:10.3390/genes14040781

 

Zhang B, Chen X, Song H, et al. Identification of basement membrane-related prognostic model associated with the immune microenvironment and synthetic therapy response in pancreatic cancer: integrated bioinformatics analysis and clinical validation. J Cancer. 2024;15(19):6273-6298. Published 2024 Oct 14. doi:10.7150/jca.100891

 

Chang SH, Jin JY, Hu YQ, Wang RY, Xiang R, Wang X. Identification of Houge type of X-linked syndromic mental retardation caused by CNKSR2 truncated variants. Ital J Pediatr. 2025;51(1):31. Published 2025 Feb 7. doi:10.1186/s13052-025-01877-0

 

Miao W, Li F, Lu J, et al. Biochar application enhanced rice biomass production and lodging resistance via promoting co-deposition of silica with hemicellulose and lignin. Sci Total Environ. 2023;855:158818. doi:10.1016/j.scitotenv.2022.158818

 

Zheng Y, Meng H, Jiang X, Huang S. Bombyx mori UFL1 facilitates BmNPV proliferation by regulating host cell apoptosis through PERK. Arch Insect Biochem Physiol. 2024;116(3):e22127. doi:10.1002/arch.22127

 

Wang XT, Liu KH, Li Y, Ren YY, Li Q, Wang BT. Zinc metalloprotease FgM35, which targets the wheat zinc-binding protein TaZnBP, contributes to the virulence of Fusarium graminearum [published correction appears in Stress Biol. 2024 Nov 27;4(1):49. doi: 10.1007/s44154-024-00200-x.]. Stress Biol. 2024;4(1):45. Published 2024 Oct 30. doi:10.1007/s44154-024-00171-z

 

Tong W, Zhong J, Yang Q, et al. Single-cell and bulk transcriptomic datasets enable the development of prognostic models based on dynamic changes in the tumor immune microenvironment in patients with hepatocellular carcinoma and portal vein tumor thrombus. Front Immunol. 2024;15:1414121. Published 2024 Oct 28. doi:10.3389/fimmu.2024.1414121

 

Qin C, Feng Y, Wang J, Huang W, Jiang L, Li Y. ER stress-related mitochondrial protein-coding gene risk model and in vitro experiments unveil OMA1 as a novel prognostic and therapeutic biomarker for low-grade glioma. Life Sci. Published online June 2, 2025. doi:10.1016/j.lfs.2025.123773

 

Jiao W, Ma D, Zuo Q, et al. SsPtc3 Modulating SsSmk1-MAPK and Autophagy to Facilitate Growth and Pathogenicity in Sclerotinia sclerotiorum. Mol Plant Pathol. 2024;25(12):e70037. doi:10.1111/mpp.70037

 

Zhang H, Luo Y, Wang Y, et al. Genome-Wide Identification and Characterization of Alternative Oxidase (AOX) Genes in Foxtail Millet (Setaria italica): Insights into Their Abiotic Stress Response. Plants (Basel). 2024;13(18):2565. Published 2024 Sep 12. doi:10.3390/plants13182565

 

Zhang Y, Lu X, Yao W, et al. Magnesium Hydride Confers Osmotic Tolerance in Mung Bean Seedlings by Promoting Ascorbate-Glutathione Cycle. Plants (Basel). 2024;13(19):2819. Published 2024 Oct 8. doi:10.3390/plants13192819

 

Ren X, Chen J, Chen S, Zhang H, Li L. Genome-Wide Identification and Characterization of CLAVATA3/EMBRYO SURROUNDING REGION (CLE) Gene Family in Foxtail Millet (Setaria italica L.). Genes (Basel). 2023;14(11):2046. Published 2023 Nov 6. doi:10.3390/genes14112046

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