English

All-in-One First-Strand Synthesis MasterMix (with dsDNase)
三代逆转录去基因组DNA一管化预混液

产品货号:
  • EG15133S
产品规格:
100 rxns
产品价格:
¥1380.00

产品介绍

       All-in-One First-Strand Synthesis MasterMix (with dsDNase) 是一款高效、便捷、减少污染的高质量一链 cDNA 合成试剂盒,包含热稳定的 M-MLV GIII Reverse Transcriptase 及其反应 Buffer、RNA 酶抑制剂、dNTPs、Oligo(dT)₂₀VN 和随机引物等一链 cDNA 合成所需的所有组分,仅需加入 RNA 模板和水即可开始反应。使用该逆转录试剂盒获得的 cDNA 主要用于下游 qPCR 实验。

       从细胞中提取的 RNA 往往存在基因组 DNA 污染,如果逆转录前不将其去除,下游 qPCR 反应时基因组 DNA 与 cDNA 会同时被扩增(尤其当引物设计在同一外显子上时),从而影响基因表达定量准确性。本试剂盒采用 dsDNase 高效去除基因组 DNA 污染,dsDNase 能够特异性消化双链 DNA(dsDNA 或 DNA-RNA 杂合链中的 DNA 链), 并且具有热敏感性,在逆转录温度下即可快速不可逆地失活。与传统 使用 DNase I 去除基因组 DNA 污染的方法相比,dsDNase 无需额外加入 EDTA 进行失活,不仅节省实验时间,而且降低了对逆转录反应的抑制。
       可依据基因组污染严重程度选择采用去基因组 DNA 污染与逆转录分开进行的操作方法,或者去基因组污染与逆转录一步法进行的操作方法。

产品组成

组分

规格

All-in-One First-Strand Synthesis MasterMix

400 μl

dsDNase

2×50 μl

10× dsDNase Buffer

200 μl

Nuclease-Free Water

2×1 ml

储运条件

-20℃保存

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Lu KK, Yang H, Liao CY, et al. A transcriptional recognition site within SOS1 coding region controls salt tolerance in Arabidopsis. Dev Cell. Published online June 6, 2025. doi:10.1016/j.devcel.2025.05.010

 

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Zhao L, Teng Q, Liu Y, et al. Machine learning-based identification of a novel prognosis-related long noncoding RNA signature for gastric cancer. Front Cell Dev Biol. 2022;10:1017767. Published 2022 Nov 11. doi:10.3389/fcell.2022.1017767

 

Liu J, Zhang B, Zhang Y, et al. Oxidative stress and autophagy-mediated immune patterns and tumor microenvironment infiltration characterization in gastric cancer. Aging (Albany NY). 2023;15(21):12513-12536. doi:10.18632/aging.205194

 

Li M, Li N, Dong Y, et al. Soil intake modifies the gut microbiota and alleviates Th2-type immune response in an ovalbumin-induced asthma mouse model. World Allergy Organ J. 2024;17(4):100897. Published 2024 Apr 14. doi:10.1016/j.waojou.2024.100897

 

Liu R, Huang Y, Li D, et al. Dihydroartemisinin alleviates diethylnitrosamine-induced hepatocarcinogenesis by targeting a novel MAZ/TRIM50 axis. Int Immunopharmacol. 2025;156:114733. doi:10.1016/j.intimp.2025.114733

 

Qin XD, Liang JF, Gan LY, et al. Blockage of polycystin-2 alleviates myocardial ischemia/reperfusion injury by inhibiting autophagy through the Ca2+/Akt/Beclin 1 pathway. Biochim Biophys Acta Mol Cell Res. 2025;1872(2):119892. doi:10.1016/j.bbamcr.2024.119892

 

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Ma Y, Wu W, Zhang Y, et al. The Synchronized Progression from Mitosis to Meiosis in Female Primordial Germ Cells between Layers and Broilers. Genes (Basel). 2023;14(4):781. Published 2023 Mar 23. doi:10.3390/genes14040781

 

Zhang B, Chen X, Song H, et al. Identification of basement membrane-related prognostic model associated with the immune microenvironment and synthetic therapy response in pancreatic cancer: integrated bioinformatics analysis and clinical validation. J Cancer. 2024;15(19):6273-6298. Published 2024 Oct 14. doi:10.7150/jca.100891

 

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Miao W, Li F, Lu J, et al. Biochar application enhanced rice biomass production and lodging resistance via promoting co-deposition of silica with hemicellulose and lignin. Sci Total Environ. 2023;855:158818. doi:10.1016/j.scitotenv.2022.158818

 

Zheng Y, Meng H, Jiang X, Huang S. Bombyx mori UFL1 facilitates BmNPV proliferation by regulating host cell apoptosis through PERK. Arch Insect Biochem Physiol. 2024;116(3):e22127. doi:10.1002/arch.22127

 

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