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快速查找限制酶工具

货号 名称 同裂酶 异裂酶 识别位点 切割末端 反应温度 失活条件 甲基化影响
GMP501S BsaI, GMP Grade Eco31I, Bso31I, BspTNI GGTCTC(1/5)

5'...G G T C T C (N)₁ ↓...3'
3'...C C A G A G (N)₅ ↑...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: impaired.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
GMP502S XbaI, GMP Grade TCTAGA

5'...T ↓ C T A G A...3'
3'...A G A T C ↑ T...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: blocked.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
GMP504S XhoI, GMP Grade PaeR7I, Sfr274I, SlaI CTCGAG

5'...C ↓ T C G A G...3'
3'...G A G C T ↑ C...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
EG15502S LightNing® AccI XmiI, FblI GTMKAC

5'...G T ↓ M K A C...3'
3'...C A K M ↑ T G...5' 		5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
EG23501S LightNing® AgeI AsiGI, CspAI, BshTI, PinAI ACCGGT

5'...A ↓ C C G G T...3'
3'...T G G C C ↑ A...5' 		5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
EG15601S LightNing® ApaLI Alw44I, VneI GTGCAC

5'...G ↓ T G C A C...3'
3'...C A C G T ↑ G...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: impaired.
EcoBI: no effect.
EG15508S LightNing® AscI PalAI, SgsI GGCGCGCC

5'...G G ↓ C G C G C C...3'
3'...C C G C G C ↑ G G...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
EG15509S LightNing® AvrII AspA2I, BlnI, XmaJI CCTAGG

5'...C ↓ C T A G G...3'
3'...G G A T C ↑ C...5'		 5’ protruding ends 37℃ No (column purification)

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG15510S LightNing® BamHI GGATCC

5'...G ↓ G A T C C...3'
3'...C C T A G ↑ G...5'		 5’ protruding ends 37℃ No (column purification)

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG15513S LightNing® BclI FbaI, Ksp22I TGATCA

5'...T ↓ G A T C A...3'
3'...A C T A G ↑ T...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: blocked.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: some blocked.
EG15591S LightNing® BglII AGATCT

5'...A ↓ G A T C T...3'
3'...T C T A G ↑ A...5'
 5’ protruding ends 37℃ No (column purification)

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: some blocked.
EG15518S LightNing® BsaI Eco31I, Bso31I, BspTNI GGTCTC(1/5)

5'...G G T C T C (N)₁ ↓...3'
3'...C C A G A G (N)₅ ↑...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: impaired.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
EG23505S LightNing® BsrGI BstAUI, Bsp1407I TGTACA

5'...T ↓ G T A C A...3'
3'...A C A T G ↑ T...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG15607S LightNing® BstBI AsuII, Bpu14I, Bsp119I, BspT104I, NspV, SfuI TTCGAA

5'...T T ↓ C G A A...3'
3'...A A G C ↑ T T...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: some blocked.
EcoBI: no effect.
EG15528S LightNing® BstEII BstPI，Eco91I，EcoO65I，PspEI GGTNACC

5'...G ↓ G T N A C C...3'
3'...C C A N T G ↑ G...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: impaired.
EcoBI: impaired.
EG15532S LightNing® ClaI BspDI，Bsa29I，BseCI，BshVI，Bsu15I，BsuTUI ATCGAT

5'...A T ↓ C G A T...3'
3'...T A G C ↑ T A...5'
 5’ protruding ends 37℃ 80℃, 20 min

Dam: blocked.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: impaired.
EG24501S LightNing® DdeI BstDEI, HpyF3I CTNAG

5'...C ↓ T N A G...3'
3'...G A N T ↑ C...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked.
EG15585S LightNing® DpnI MalI Bsp143I, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI GAᵐ⁶TC

5'...G Aᵐ⁶ ↓ T C...3'
3'...C T ↑ Aᵐ⁶ G...5'		 blunt 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: impaired.
EG15533S LightNing® DpnII MboI，Sau3AI，Bsp143I, BssMI，BstMBI，Kzo9I，NdeII BstKTI, DpnI, MalI GATC

5'...↓ G A T C...3'
3'...C T A G ↑...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: blocked.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked.
EG23502S LightNing® DraI TTTAAA

5'...T T T ↓ A A A...3'
3'...A A A ↑ T T T ..5'		 blunt 37℃ 80℃，20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: blocked.
EcoBI: no effect.
EG15535S LightNing® EagI BseX3I, BstZI, EclXI, Eco52I CGGCCG

5'...C ↓ G G C C G...3'
3'...G C C G G ↑ C ..5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
EG23511S LightNing® EarI Eam1104I, Bst6I CTCTTC(1/4)

5'...C T C T T C (N)₁↓...3'
3'...G A G A A G (N)₄↑...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
EG15536S LightNing® EcoRI FunII GAATTC

5'...G ↓ A A T T C...3'
3'...C T T A A ↑ G...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: impaired.
EG15537S LightNing® EcoRV Eco32I GATATC

5'...G A T ↓ A T C...3'
3'...C T A ↑ T A G...5'		 blunt 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: some impaired.
EcoKI: no effect.
EcoBI: some blocked.
EG21503S LightNing® Esp3I (BsmBI) BsmBI CGTCTC(1/5)

5'...C G T C T C (N)₁↓...3'
3'...G C A G A G (N)₅↑...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
EG15586S LightNing® FspI Acc16I,NsbI TGCGCA

5'...T G C ↓ G C A...3'
3'...A C G ↑ C G T...5'		 blunt 37℃ No (column purification)

Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
EG23509S LightNing® HaeIII BsuRI, BshFI, BspANI, BsnI AoxI GGCC

5'...G G ↓ C C...3'
3'...C C ↑ G G...5'		 blunt 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG15539S LightNing® HindIII AAGCTT

5'...A ↓ A G C T T...3'
3'...T T C G A ↑ A...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: some blocked.
EG15593S LightNing® HinfI GANTC

5'...G ↓ A N T C...3'
3'...C T N A ↑ G...5'
 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: blocked.
EG15541S LightNing® HpaI KspAI GTTAAC

5'...G T T ↓ A A C...3'
3'...C A A ↑ T T G...5'		 blunt 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: impaired.
EcoBI: no effect.
EG23507S LightNing® HpaII MspI, HapII, BsiSI CCGG

5'...C ↓ C G G...3'
3'...G G C ↑ C...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
EG15543S LightNing® KasI SspDI Mly113I, EgeI, NarI, EheI, DinI, SfoI, PluTI GGCGCC

5'...G ↓ G C G C C...3'
3'...C C G C G ↑ G...5'		 blunt 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
EG15544S LightNing® KpnI Asp718I, Acc65I GGTACC

5'...G G T A C ↓ C...3'
3'...C ↑ C A T G G...5'		 3’ protruding ends 37℃ No (column purification)

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG15547S LightNing® MluI ACGCGT

5'...A ↓ C G C G T...3'
3'...T G C G C ↑ A...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: impaired.
EcoBI: impaired.
EG15548S LightNing® MnlI CCTC(7/6)

5'...C C T C N₇ ↓...3'
3'...G G A G N₆ ↑...5'		 3’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked.
EG23508S LightNing® MspI HpaII, HapII, BsiSI CCGG

5'...C ↓ C G G...3'
3'...G G C ↑ C...5'		 5’ protruding ends 37℃ No (column purification)

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG23510S LightNing® MunI (MfeI) MfeI CAATTG

5'...C ↓ A A T T G...3'
3'...G T T A A ↑ C...5'		 5’ protruding ends 37℃ No (column purification)

EG15550S LightNing® NcoI Bsp19I CCATGG

5'...C ↓ C A T G G...3'
3'...G G T A C ↑ C...5'
 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG15551S LightNing® NdeI FauNDI CATATG

5'...C A ↓ T A T G...3'
3'...G T A T A ↑ C...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG15552S LightNing® NheI AsuNHI BmtI, BspOI GCTAGC

5'...G ↓ C T A G C...3'
3'...C G A T C ↑ G...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: some blocked.
EcoKI: no effect.
EcoBI: no effect.
EG15553S LightNing® NotI CciNI GCGGCCGC

5'...G C ↓ G G C C G C...3'
3'...C G C C G G ↑ C G...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
EG15602S LightNing® NruI Bsp68I，BtuMI，RruI TCGCGA

5'...T C G ↓ C G A...3'
3'...A G C ↑ G C T...5'		 blunt 37℃ No (column purification)

Dam: blocked.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
EG15606S LightNing® NsiI EcoT22I, Mph1103I, Zsp2I ATGCAT

5'...A T G C A ↓ T...3'
3'...T ↑ A C G T A...5'		 3’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: some impaired.
EG15603S LightNing® PacI TTAATTAA

5'...T T A A T ↓ T A A...3'
3'...A A T ↑ T A A T T...5'
 3’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG23506S LightNing® PmeI MssI GTTTAAAC

5'...G T T T ↓ A A A C...3'
3'...C A A A ↑ T T T G...5'		 blunt 37℃ 80℃,20 min

Dam: no effect.
Dcm: no effect.
CpG: some blocked.
EcoKI: no effect.
EcoBI: no effect.
EG15560S LightNing® PspGI (EcoRII) EcoRII, Psp6I, AjnI BciT130I, BseBI, Bst2UI, BstNI, MvaI CCWGG

5'... ↓ C C W G G ...3'
3'... G G W C C ↑ ...5'		 5’ protruding ends 75℃ No (column purification)

Dam: no effect.
Dcm: blocked.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG15561S LightNing® PstI BspMAI CTGCAG

5'...C T G C A ↓ G...3'
3'...G ↑ A C G T C...5'		 3’ protruding ends 37℃ No (column purification)

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG23513S LightNing® PvuI Ple19I CGATCG

5'...C G A T ↓ C G...3'
3'...G C ↑ T A G C...5' 		3’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
EG15604S LightNing® PvuII CAGCTG

5'...C A G ↓ C T G...3'
3'...G T C ↑ G A C...5'		 blunt 37℃ No (column purification)

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: impaired.
EG24503S LightNing® RsaI AfaI Csp6I, CviQI, RsaNI GTAC

5'...G T ↓ A C...3'
3'...C A ↑ T G...5'		 blunt 37℃ No (column purification)

Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
EG15565S LightNing® SacI Psp124BI, SstI Ecl136II, EcoICRI, Eco53kI GAGCTC

5'...G A G C T ↓ C...3'
3'...C ↑ T C G A G...5'		 3’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG15605S LightNing® SacII Cfr42I，KspI，Sfr303I，SgrBI CCGCGG

5'...C C G C ↓ G G...3'
3'...G G ↑ C G C C...5'		 3’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
EG15566S LightNing® SalI GTCGAC

5'...G ↓ T C G A C...3'
3'...C A G C T ↑ G...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: some blocked.
EcoKI: no effect.
EcoBI: no effect.
EG15568S LightNing® SbfI SdaI, Sse8387I CCTGCAGG

5'...C C T G C A ↓ G G...3'
3'...G G ↑ A C G T C C...5'		 3’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG24502S LightNing® ScaI ZrmI, BmcAI AGTACT

5'...A G T ↓ A C T...3'
3'...T C A ↑ T G A...5'		 blunt 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked.
EG15570S LightNing® SfiI GGCCNNNNNGGCC

5'...G G C C N N N N ↓ N G G C C...3'
3'...C C G G N ↑ N N N N C C G G...5'		 3’ protruding ends 50℃ No (column purification)

Dam: no effect.
Dcm: impaired.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
EG15572S LightNing® SmaI Cfr9I, TspMI, XmaI CCCGGG

5'...C C C ↓ G G G...3'
3'...G G G ↑ C C C...5'		 blunt 25℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EcoBI: no effect.
EG15574S LightNing® SpeI AhlI，BcuI ACTAGT

5'...A ↓ C T A G T...3'
3'...T G A T C ↑ A...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: blocked.
EcoBI: blocked.
EG15575S LightNing® SphI PaeI GCATGC

5'...G C A T G ↓ C...3'
3'...C ↑ G T A C G...5'		 3’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: blocked.
EG15576S LightNing® SspI AATATT

5'...A A T ↓ A T T...3'
3'...T T A ↑ T A A...5'		 blunt 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG15577S LightNing® StuI Eco147I, PceI, SseBI AGGCCT

5'...A G G ↓ C C T...3'
3'...T C C ↑ G G A...5'		 blunt 37℃ 80℃, 20 min

Dam: no effect.
Dcm: blocked.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG15580S LightNing® TaqI TCGA

5'...T ↓ C G A...3'
3'...A G C ↑ T...5'		 5’ protruding ends 65℃ No (column purification)

Dam: blocked.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG15581S LightNing® XbaI TCTAGA

5'...T ↓ C T A G A...3'
3'...A G A T C ↑ T...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: blocked.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG15582S LightNing® XcmI CCANNNNNNNNNTGG

5'...C C A N N N N N ↓ N N N N T G G...3'
3'...G G T N N N N ↑ N N N N N A C C...5'		 3’ protruding ends 37℃ 80℃，20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG15583S LightNing® XhoI PaeR7I, Sfr274I, SlaI CTCGAG

5'...C ↓ T C G A G...3'
3'...G A G C T ↑ C...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
EG22507V BsmBI Esp3I CGTCTC(1/5)

5'...C G T C T C (N)₁↓...3'
3'...G C A G A G (N)₅↑...5'		 5’ protruding ends 55℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: some blocked.
EcoKI: no effect.
EcoBI: impaired.
EG23503S BspQI SapI, LguI, PciSI GCTCTTC(1/4)

5'...G C T C T T C (N)₁↓...3'
3'...C G A G A A G (N)₄↑...5'		 5’ protruding ends 50℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG21501S Sgel m⁵CNNG(9/13)

5'...m⁵ C N N G(N)₉ ↓...3'
3'... G N N C(N)₁₃ ↑...5'*
*SgeI 可识别与切割含有5-mC位点的DNA靶标序列，一条链或双链甲基化均可被识别。		 3’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effct.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG21504S Esp3I(BsmBI), ADCF BsmBI CGTCTC(1/5)

5'...C G T C T C (N)₁...3'
3'...G C A G A G (N)₅...5'
 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: blocked.
EcoKI: no effect.
EG22504S EcoRI, ADCF FunII, LraI, RsrI, SsoI GAATTC

5'...G ↓ A A T T C...3'
3'...C T T A A ↑ G...5'		 5’ protruding ends 37℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: impaired.
EcoKI: no effect.
EcoBI: no effect.
EG23514S Nb.BsrDI GCAATGNN

5'...G C A A T G N N...3'
3'...C G T T A C↑N N ...5'		 65℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG23512S Nt.BspQI GCTCTTCN

5'...G C T C T T C N↓...3'
3'...C G A G A A G N ...5'		 50℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.
EG24504S Nt.BstNBI GAGTCNNNNN

5'...G A G T C N N N N N↓...3'
3'...C T C A G N N N N N ...5'		 55℃ 80℃, 20 min

Dam: no effect.
Dcm: no effect.
CpG: no effect.
EcoKI: no effect.
EcoBI: no effect.

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